| Autor: |
D A, Bochar, L, Wang, H, Beniya, A, Kinev, Y, Xue, W S, Lane, W, Wang, F, Kashanchi, R, Shiekhattar |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Cell. 102(2) |
| ISSN: |
0092-8674 |
| Popis: |
Germline mutations in the tumor suppressor gene, BRCA1, predispose individuals to breast and ovarian cancers. Using a combination of affinity- and conventional chromatographic techniques, we have isolated a predominant form of a multiprotein BRCA1-containing complex from human cells displaying chromatin-remodeling activity. Mass spectrometric sequencing of components of this complex indicated that BRCA1 is associated with a SWI/SNF-related complex. We show that BRCA1 can directly interact with the BRG1 subunit of the SWI/SNF complex. Moreover, p53-mediated stimulation of transcription by BRCA1 was completely abrogated by either a dominant-negative mutant of BRG1 or the cancer-causing deletion in exon 11 of BRCA1. These findings reveal a direct function for BRCA1 in transcriptional control through modulation of chromatin structure. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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