| Autor: |
Yi, Xie, Zi-Li, Fan, Chen-Jiao, Yao, San-Qin, Tan, Ya-Li, Zhao |
| Rok vydání: |
2006 |
| Předmět: |
|
| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 14(2) |
| ISSN: |
1009-2137 |
| Popis: |
The study was aimed to investigate the expression of VEGF mRNA and VEGF protein in HL-60 cells treated with diallyl disulfide (DADS), and to explore the antileukemic mechanism of DADS in respect of VEGF production. Semi-quantitative RT-PCR and ELISA were used to detect the expression of VEGF mRNA and secretion of VEGF protein in HL-60 cell lines treated by DADS respectively. The results showed that the expression of VEGF mRNA and secretion of VEGF protein were found in HL-60 cells. The expression of VEGF mRNA and secretion of VEGF protein in HL-60 cells could be down regulated by treatment with 0.625, 1.25, and 2.5 microg/mL DADS for 48 and 72 hours and the effects had a dose dependent relationship (r0.9, P0.01). The differences between DADS treated HL-60 cell groups and the control group were statistically significant (P0.01), there were also statistically significant differences among three DADS-treated HL-60 cell groups (P0.05). It is concluded that DADS effectively inhibits the proliferation of human leukemia cell line HL-60 cells; DADS exerts its antileukemic effects by reduction of the expression of VEGF mRNA and VEGF protein secretion. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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