Involvement of CD28 in MHC-unrestricted cytotoxicity mediated by a human natural killer leukemia cell line
| Autor: | M, Azuma, M, Cayabyab, D, Buck, J H, Phillips, L L, Lanier |
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| Rok vydání: | 1992 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Cytotoxicity Immunologic B-Lymphocytes Intercellular Adhesion Molecule-1 Lymphocyte Function-Associated Antigen-1 Killer Cells Natural Major Histocompatibility Complex CD28 Antigens Antigens CD Antigens Surface B7-1 Antigen Tumor Cells Cultured Humans Cell Adhesion Molecules |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 149(4) |
| ISSN: | 0022-1767 |
| Popis: | NK cells and certain CTL can recognize and lyse targets without restriction by the MHC. NK cells do not express CD3/TCR complexes and the membrane receptors participating in MHC-unrestricted cytotoxicity are largely unknown. We demonstrate that YT2C2, a human NK leukemia cell line, expresses the CD28 differentiation Ag and can spontaneously lyse both murine and human cell lines expressing B7, a B cell- activation Ag that is a ligand for CD28. The participation of CD28/B7 interactions in MHC-unrestricted cytotoxicity mediated by YT2C2 cells was demonstrated by correlation of target sensitivity with levels of B7 expression, inhibition of cytotoxicity by anti-CD28 or anti-B7 mAb, and by making both murine and human cell lines susceptible to YT2C2-mediated lysis by genetic transfection with expression vectors containing B7 cDNA. However, CD28/B7 interactions alone were insufficient to initiate cytotoxicity. mAb inhibition experiments and selection of CD54- (intercellular adhesion molecule-1) deficient B cell targets indicated that CD11a/18 (lymphocyte function-associated Ag-1) also cooperated in CD28/B7-dependent cytotoxicity. The requirement for both CD28/B7 and lymphocyte function-associated Ag-1/intercellular adhesion molecule-1 interactions in YT2C2-mediated MHC-unrestricted cytotoxicity was confirmed by demonstrating that efficient lysis of murine L cells required cotransfection with both B7 and intercellular adhesion molecule-1. These findings support the concept that MHC-unrestricted cytotoxicity may not be due to a unique receptor, but may result from interactions between an appropriate array of "adhesion" molecules with their ligands. |
| Databáze: | OpenAIRE |
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