In vivo visualization of osteoarthritic hypertrophic lesions† †Electronic supplementary information (ESI) available: Supplemental figures, synthetic schemes, experimental procedures, characterization of all new compounds, histopathological scoring of joint damage in the rat ACLT-pMx OA model, detailed ex vivoMRI studies with pig cartilage explants, in vivo MRI studies with rats, immunofluorescence staining for procollagen type IIA. See DOI: 10.1039/c5sc01301a

Autor: Hu, Hai-Yu, Lim, Ngee-Han, Juretschke, Hans-Paul, Ding-Pfennigdorff, Danping, Florian, Peter, Kohlmann, Markus, Kandira, Abdullah, Peter von Kries, Jens, Saas, Joachim, Rudolphi, Karl A., Wendt, K. Ulrich, Nagase, Hideaki, Plettenburg, Oliver, Nazare, Marc, Schultz, Carsten
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Chemical Science
ISSN: 2041-6539
2041-6520
Popis: Cartilage-binding bimodal MRI and fluorescent probes were developed to monitor osteoarthritic damage in animal models over extended periods of time.
Osteoarthritis (OA) is one of the most common diseases in the aging population. While disease progress in humans is monitored indirectly by X-ray or MRI, small animal OA lesions detection always requires surgical intervention and histology. Here we introduce bimodal MR/NIR probes based on cartilage-targeting 1,4,7,10-tetraazacyclododecane 1,4,7,10-tetraacetic acid amide (DOTAM) that are directly administered to the joint cavity. We demonstrate applications in healthy and diseased rat joints by MRI in vivo. The same joints are inspected post-mortem by fluorescence microscopy, showing not only the precise location of the reagents but also revealing details such as focal cartilage damage and chondrophyte or osteophyte formation. This allows for determining the distinct pathological state of the disease and the regeneration capability of the animal model and will help to correctly assess the effect of potential disease modifying OA drugs (DMOADs) in the future.
Databáze: OpenAIRE
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