| Autor: |
Cristina, Velasquillo, Phaedra, Silva-Bermudez, Nadia, Vázquez, Alan, Martínez, Andres, Espadín, Julieta, García-López, Antonio, Medina-Vega, Hugo, Lecona, Raúl, Pichardo-Baena, Clemente, Ibarra, Keiko, Shirai |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Journal of biomedical materials research. Part A. 105(10) |
| ISSN: |
1552-4965 |
| Popis: |
Autologous skin transplantation is today's "gold standard" treatment for full-thickness burns. However, when 30% of total body surface area is damaged, there is an important shortage of autologous donor sites for skin grafting; then, treatment alternatives become crucial. Such alternatives can be based on polymeric scaffolds capable of functioning as protective covers and cells/factors carriers. Chitosan (CTS) is a natural-derived polymer with relevant biological-related properties but poor mechanical performance. Improved mechanical properties can be achieved through lactic acid grafting (LA-g); nevertheless, LA-g affects the biological response towards the CTS-based materials. In this work, CTS-LA scaffolds with different LA-g percentages were synthesized and evaluated to determine appropriate LA-g degrees for full-thickness burns treatment. In vitro results indicated that the higher the LA-g percentage, the lower the capability of the scaffolds to sustain fibroblasts culture. Scaffolds with LA-g around 28% (CTS-LA28) sustained cell culture and allowed normal cell functionality. Further evaluation of CTS-LA28 as acellular and cellular grafts in a full-thickness burn mouse model showed that at 28 days post-burn, macroscopic characteristic of the reparation tissue were closer to healthy skin when cellular grafts were used for treatment; histological evaluation also showed that dermis cellularity and collagenous fibers structure were similar to those in healthy skin when cellular grafts were used for burns treatment. © 2017 Wiley Periodicals Inc. J Biomed Mater Res Part A: 105A: 2875-2891, 2017. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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