| Autor: |
M G, Davey, H L, Hedrick, S, Bouchard, N S, Adzick, A W, Flake, E J, Doolin |
| Rok vydání: |
2003 |
| Předmět: |
|
| Zdroj: |
Scanning. 25(1) |
| ISSN: |
0161-0457 |
| Popis: |
The aim of this study was to determine the accuracy of a new computer-assisted stereological technique in obtaining structural information of the lung. We compared the point fraction of lung parenchyma (Pp) and alveolar surface density (Sv) obtained by established manual point/intercept counting methods and compared them with those obtained using a computer-assisted method. Lung tissues obtained from normally grown fetal sheep (n = 6) and from newborn lambs with severe lung hypoplasia (n = 5) were inflation fixed via the trachea and processed for light microscopy. In verification-of-technique experiments, Pp and Sv correlated well with known values. There was a significant linear correlation between manual and computer-assisted stereological measurements for values of Pp (r2 = 0.92) and Sv (r2 = 0.98). Our data lead us to believe that the computer-assisted stereological technique described in this study provides accurate estimates of Pp and Sv and hence may be a valuable tool for evaluating the effects of factors upon structural development of the lung. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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