Highly sensitive northern hybridization using a rapid protocol for downward alkaline blotting of RNA

Autor:	I L, Ingelbrecht, C I, Mandelbaum, T E, Mirkov
Rok vydání:	1998
Předmět:	Capsid RNA RNA Viral Sodium Hydroxide Transgenes Plants Blotting Northern Sensitivity and Specificity
Zdroj:	BioTechniques. 25(3)
ISSN:	0736-6205
Popis:	A simple and fast RNA gel blot procedure is described that uses 50 mM NaOH to simultaneously transfer and fix RNA to a positively charged nylon membrane. The RNA is transferred in a downward direction, and transfer is routinely completed within 2.5 h. The resulting blots give increased sensitivity over existing methods without affecting RNA integrity and can be used in both radioactive and nonradioactive detection procedures.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::d3f786738536f3f5aabb9a2ef04ac163 https://pubmed.ncbi.nlm.nih.gov/9762439 Zobrazit plný text záznamu