The multigene family of the tobacco hornworm V-ATPase: novel subunits a, C, D, H, and putative isoforms
| Autor: | H, Merzendorfer, S, Reineke, X F, Zhao, B, Jacobmeier, W R, Harvey, H, Wieczorek |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Vacuolar Proton-Translocating ATPases
DNA Complementary Base Sequence Sequence Homology Amino Acid Molecular Sequence Data Gene Expression Genes Insect Isoenzymes Proton-Translocating ATPases Manduca Multigene Family Animals Amino Acid Sequence Cloning Molecular Protein Structure Quaternary DNA Primers |
| Zdroj: | Biochimica et biophysica acta. 1467(2) |
| ISSN: | 0006-3002 |
| Popis: | The plasma membrane V-ATPase from Manduca sexta (Lepidoptera, Sphingidae) larval midgut is composed of at least 12 subunits, eight of which have already been identified molecularly [Wieczorek et al., J. Bioenerg. Biomembr. 31 (1999) 67-74]. Here we report primary sequences of subunits C, D, H and a, which previously had not been identified in insects. Expression of recombinant proteins, immunostaining and protein sequencing demonstrated that the corresponding proteins are subunits of the Manduca V-ATPase. Genomic Southern blot analysis indicated the existence of multiple genes encoding subunits G, a, c, d and e. Moreover, multiple transcripts were detected in Northern blots from midgut poly(A) RNA for subunits B, G, c and d. Thus, these polypeptides appear to exist as multiple isoforms that could be expressed either in different tissues or at distinct locations within a cell. By contrast subunits A, C, D, E, F and H appear to be encoded by single transcripts and therefore should be present in any Manduca V-ATPase, independent of its subcellular or cell specific origin. |
| Databáze: | OpenAIRE |
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