Characterization of the Streptococcus sobrinus acid-stress response by interspecies microarrays and proteomics
| Autor: | A R, Martinez, J, Abranches, J K, Kajfasz, J A, Lemos |
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| Rok vydání: | 2010 |
| Předmět: |
Streptococcus sobrinus
Gene Expression Profiling Nucleic Acid Hybridization Gene Expression Regulation Bacterial Hydrogen-Ion Concentration Adaptation Physiological Article Streptococcus mutans stomatognathic diseases Bacterial Proteins Species Specificity Malate Dehydrogenase Tandem Mass Spectrometry Lactic Acid Glycolysis Oligonucleotide Array Sequence Analysis |
| Zdroj: | Molecular oral microbiology. 25(5) |
| ISSN: | 2041-1014 |
| Popis: | Streptococcus mutans and Streptococcus sobrinus are considered the primary organisms responsible for human dental caries. The ability to generate acids and to adapt to low pH conditions is directly associated with the cariogenic potential of these bacteria. To survive acidic conditions, both species have been shown to mount an acid tolerance response (ATR). However, previous characterization of the S. sobrinus ATR identified critical differences in the mechanisms of acid adaptation between S. mutans and S. sobrinus. Here, interspecies microarray and proteomic approaches were used to identify novel, previously unrecognized genes and pathways that participate in the S. sobrinus acid stress response. The results revealed that, among other things, metabolic alterations that enhance energy generation and upregulation of the malolactic fermentation enzyme activity constitute important acid resistance properties in S. sobrinus. Some of these acid adaptive traits are shared by S. mutans and might be considered optimal targets for therapeutic treatments designed to control dental caries. |
| Databáze: | OpenAIRE |
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