[The determination of the genotype of natural reassortant influenza A viruses according to the core protein genes by the methods of competitive dot hybridization and sequencing]

Autor:	E B, Grinbaum, F N, Zolotarev, N A, Petrov, O M, Litvinova, I B, Konovalenko, T Ia, Luzianina, D B, Golubev
Rok vydání:	1992
Předmět:	Genotype Influenza A virus Viral Core Proteins DNA Viral Nucleic Acid Hybridization DNA Sequence Analysis DNA Serotyping Antigens Viral Reassortant Viruses Plasmids
Zdroj:	Voprosy virusologii. 37(5-6)
ISSN:	0507-4088
Popis:	Simultaneous circulation of different subtypes of influenza A viruses provides conditions for reassortant strains formation. A comparative investigation of genome of 47 influenza A virus strains (H1N1, H2N2, and H3N2) was carried out by competitive dot hybridization technique and sequence analysis of some of cDNA-copies of the virus genes. All the genes of 43 strains encoding nonglycolysed proteins corresponded to the serum subtype of surface glycoproteins. The reassortant pattern of genome for some genes of core proteins was revealed in 4 viruses. All the dot hybridization data were completely confirmed by sequence analysis of the genes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::d35a87b12f65d1dbd8baaf0d1c7a52a6 https://pubmed.ncbi.nlm.nih.gov/1290222 Zobrazit plný text záznamu