| Autor: |
Li, Zhao, Jing, Li, Yan Hui, Hao, Ya Bing, Gao, Shui Ming, Wang, Jing, Zhang, Ji, Dong, Hong Mei, Zhou, Shu Chen, Liu, Rui Yun, Peng |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Biomedical and environmental sciences : BES. 30(5) |
| ISSN: |
0895-3988 |
| Popis: |
To investigate microwave-induced morphological and functional injury of natural killer (NK) cells and uncover their mechanisms.NK-92 cells were exposed to 10, 30, and 50 mW/cm2 microwaves for 5 min. Ultrastructural changes, cellular apoptosis and cell cycle regulation were detected at 1 h and 24 h after exposure. Cytotoxic activity was assayed at 1 h after exposure, while perforin and NKG2D expression were detected at 1 h, 6 h, and 12 h after exposure. To clarify the mechanisms, phosphorylated ERK (p-ERK) was detected at 1 h after exposure. Moreover, microwave-induced cellular apoptosis and cell cycle regulation were analyzed after blockade of ERK signaling by using U0126.Microwave-induced morphological and ultrastructural injury, dose-dependent apoptosis (P0.001) and cell cycle arrest (P0.001) were detected at 1 h after microwave exposure. Moreover, significant apoptosis was still detected at 24 h after 50 mW/cm2 microwave exposure (P0.01). In the 30 mW/cm2 microwave exposure model, microwaves impaired the cytotoxic activity of NK-92 cells at 1 h and down regulated perforin protein both at 1 h and 6 h after exposure (P0.05). Furthermore, p-ERK was down regulated at 1 h after exposure (P0.05), while ERK blockade significantly promoted microwave-induced apoptosis (P0.05) and downregulation of perforin (P0.01).Microwave dose-dependently induced morphological and functional injury in NK-92 cells, possibly through ERK-mediated regulation of apoptosis and perforin expression. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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