| Autor: |
D T, Le, K J, Donnelly, N, Wu, B K, Sevilla, S I, Rapaport |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
Thrombosis and haemostasis. 77(3) |
| ISSN: |
0340-6245 |
| Popis: |
The original tissue factor-dependent factor V assay for activated protein C resistant factor Va (Blood 1995; 85: 1704-1711) has been modified to use a calcium containing thromboplastin and to express results as an observed to expected ratio (Obs/Exp.). The latter permits establishing a normal range independent of variations due to differences in reagents. Comparing Obs/Exp ratios with DNA analysis in 72 persons revealed that an Obs/Exp ratio of 0.6 distinguished without overlap normals from heterozygotes for FV R506Q. Three homozygotes had a ratio of0.1. Application of this Obs/Exp cut-off ratio of 0.6 to a total of 226 plasma samples tested to date discriminated without overlap between normals and heterozygotes. We conclude that this assay-readily adaptable to any dedicated coagulation laboratory and capable of yielding reliable results in all clinical circumstances in which testing is indicated-can distinguish between normals and heterozygotes for the FV R506Q mutation without the need for confirmatory DNA analysis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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