[Intergeneric Escherichia coli-Streptomyces conjugation as a means for the transfer of conjugative plasmids into producers of antibiotics chlortetracycline and bialaphos]

Autor:	V Iu, Tabakov, T A, Voeĭkova, I L, Tokmakova, A P, Bolotin, E Iu, Vavilova, n D, Lomovskaia
Rok vydání:	1994
Předmět:	Organophosphorus Compounds Conjugation Genetic Escherichia coli Streptomyces Anti-Bacterial Agents Chlortetracycline Plasmids
Zdroj:	Genetika. 30(1)
ISSN:	0016-6758
Popis:	A system for introduction of plasmids into industrial producers of antibiotics chlortetracycline and bialaphos using intergeneric conjugation of Escherichia coli and Streptomyces was developed. Low level stability of inheritance of autonomously replicating DNA in recipient strains was shown. Site-specific integration of the conjugative-integrative vector pTO1 provided stable plasmid maintenance within the chromosomes of Streptomyces aureofaciens and S. hygroscopicus. Phenomenon of disturbance in differentiation and antibiotic production, resulting from pTO1 integration into S. hygroscopicus chromosome, was discovered.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::d2834dd1093bf1a413eae01a1b38af8b https://pubmed.ncbi.nlm.nih.gov/8188047 Zobrazit plný text záznamu