RNAi screen for kinases and phosphatases that play a role in antigen presentation by dendritic cells

Autor:	Catarina F, Moita, Ângelo, Chora, Nir, Hacohen, Luis F, Moita
Rok vydání:	2011
Předmět:	Antigen Presentation Blotting Western Phosphotransferases Mice Transgenic Dendritic Cells Flow Cytometry Immunity Innate Phosphoric Monoester Hydrolases Mice Inbred C57BL Mice Cross-Priming Animals RNA RNA Interference Gene Silencing Activin Receptors Type I
Zdroj:	European journal of immunology. 42(7)
ISSN:	1521-4141
Popis:	Effective CD8(+) T-cell responses against tumor or microbial antigens that are not directly expressed in antigen-presenting cells (APCs) depend on the cross-presentation of these antigens on MHC class I in APCs. To identify signaling molecules that regulate cross-presentation, we used lentiviral-based RNA interference to test the roles of hundreds of kinases and phosphatases in this process. Our study uncovered eight previously unknown genes, consisting of one positive and seven negative regulators of antigen cross-presentation. Depletion of Acvr1c, a type I receptor for TGF-β family of signaling molecules, led to an increase in CD80 and CD86 co-stimulator surface expression and secreted IL-12 in mouse bone marrow-derived DCs, as well as antigen-specific T-cell proliferation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::d261c3b0032b383c225f2410b8537e89 https://pubmed.ncbi.nlm.nih.gov/22585713 Zobrazit plný text záznamu Plný text