| Autor: |
Yumei, Wang, Qian, Fan, Jun, Xiang, Haibo, Huang, Sheng, Chen, Bairu, Liu, Aizhi, Wu, Cuixian, Zhang, Li, Rong |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Rapid communications in mass spectrometry : RCMREFERENCES. 34(11) |
| ISSN: |
1097-0231 |
| Popis: |
Paris polyphylla var. yunnanensis (Franch) Hand Mazz (PPY) is a traditional Chinese medicine with antitumor, antibacterial, hemostatic, and anthelmintic activities. Identification of the chemical composition in PPY is helpful to discover its active ingredients and can be used to establish its quality control protocols.The composition of PPY was identified using ultra-high-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS/MS) coupled with a molecular networking strategy. First, the UHPLC/QTOF-MS/MS approach was optimized for chemical compound profiling. Then, the MS data were processed using PeakView™ combined with an in-house database to quickly characterize the secondary metabolites. Finally, molecular networking excavated new molecular weights to discover unknown or trace natural products based on the characteristics of each cluster.A total of 222 compounds, including 77 isospirostanols, 2 spirostanols, 19 furostanols, 10 pseudospirostanols, 6 cholesterols, 10 CThis study extended the application of a molecular networking strategy to traditional herbal medicines and developed a molecular networking based screening approach with a significant increase in efficiency for the discovery and identification of trace novel natural products. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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