Zebrafish homolog of the leukemia gene CBFB: its expression during embryogenesis and its relationship to scl and gata-1 in hematopoiesis
| Autor: | T, Blake, N, Adya, C H, Kim, A C, Oates, L, Zon, A, Chitnis, B M, Weinstein, P P, Liu |
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| Rok vydání: | 2000 |
| Předmět: |
DNA
Complementary Embryo Nonmammalian Molecular Sequence Data Kidney Core Binding Factor beta Subunit Mesoderm Mice Species Specificity Proto-Oncogene Proteins Basic Helix-Loop-Helix Transcription Factors Animals Humans GATA1 Transcription Factor Amino Acid Sequence T-Cell Acute Lymphocytic Leukemia Protein 1 Zebrafish Leukemia Sequence Homology Amino Acid Core Binding Factor alpha Subunits Gene Expression Regulation Developmental DNA Zebrafish Proteins Hematopoiesis DNA-Binding Proteins Genes Transcription Factor AP-2 Organ Specificity Chromosome Inversion Erythroid-Specific DNA-Binding Factors Sequence Alignment Chromosomes Human Pair 16 Protein Binding Transcription Factors |
| Zdroj: | Blood. 96(13) |
| ISSN: | 0006-4971 |
| Popis: | Mammalian CBFB encodes a transcription factor (CBF beta) that in combination with CBF alpha 2 binds to specific DNA sequences and regulates expression of a number of hematopoietic genes. CBFB is associated with human leukemias through a chromosome 16 inversion and is essential for definitive hematopoiesis during mouse embryo development. We have isolated a zebrafish cbfb complementary DNA (cDNA) clone from a zebrafish kidney cDNA library. This cbfb is highly homologous to human and mouse CBFB/Cbfb genes at both the DNA and protein level. In biochemical analyses, cbfbeta binds to human CBF alpha 2 and enhances its DNA binding. During zebrafish development, cbfb is expressed in the lateral plate mesoderm at tail bud stage and in the intermediate cell mass (ICM, the location of embryonic hematopoiesis) between the 21- to 26-somite stages. The cbfb is also expressed in Rohon-Beard cells, cranial nerve ganglia, hindbrain, retina, branchial arches, jaw, and fin buds. Expression of cbfb is decreased or absent in the ICM and Rohon-Beard cells in some hematopoietic mutants and is unaffected in others. We have also analyzed the expression of scl and gata-1 in the same hematopoietic mutants to ascertain the relative order of these transcription factors to cbfb in zebrafish hematopoiesis. Our results indicate that cbfb is expressed in early hematopoietic progenitors and that its expression pattern in the hematopoietic mutants is similar to that of scl. (Blood. 2000;96:4178-4184) |
| Databáze: | OpenAIRE |
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