Searching for depolarization-induced genes that modulate synaptic plasticity and neurotrophin-induced genes that mediate neuronal differentiation

Autor:	H R, Herschman, G D, Ferguson, J D, Feldman, R, Farias-Eisner, L, Vician
Rok vydání:	2000
Předmět:	Male Neurons Kainic Acid Neuronal Plasticity Epidermal Growth Factor Colforsin Adrenal Gland Neoplasms Cell Differentiation Pheochromocytoma PC12 Cells Rats Gene Expression Regulation Synapses Animals Nerve Growth Factors Genes Immediate-Early
Zdroj:	Neurochemical research. 25(5)
ISSN:	0364-3190
Popis:	We identify and characterize two classes of immediate-early genes: (i) genes, induced by depolarization in neurons, that play a role in depolarization-induced neuronal plasticity and (ii) genes, induced in neuronal precursors by neurotrophins, that play a causal role in neurotrophin-directed neuronal differentiation. We use rat PC12 pheochromocytoma cells to identify (i) genes preferentially induced by [depolarization or forskolin] versus [Nerve Growth Factor (NGF) or Epidermal Growth Factor (EGF)] and (ii) genes preferentially induced by NGF versus EGF. We describe (i) a collection of genes preferentially induced by depolarization/forskolin in PC12 cells and by kainic acid in vivo, and (ii) a collection of genes preferentially induced by NGF. The synaptotagmin IV gene encodes a synaptic vesicle protein whose level is modulated by depolarization. NGF preferentially induces the urokinase-plasminogen activator receptor in PC12 cells. Antisense oligonucleotide and anti-UPAR antibody experiments demonstrate that NGF-induced UPAR expression is required for NGF-driven PC12 cell differentiation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::d1d5ee22cf98af3b8c17467a06d331e3 https://pubmed.ncbi.nlm.nih.gov/10905620 Zobrazit plný text záznamu Full text from SpringerLink