Polymorphism in the
| Autor: | Catherine, Daniel, Amélie, Dewitte, Sabine, Poiret, Michaël, Marceau, Michel, Simonet, Laure, Marceau, Guillaume, Descombes, Denise, Boutillier, Nadia, Bennaceur, Sébastien, Bontemps-Gallo, Nadine, Lemaître, Florent, Sebbane |
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| Rok vydání: | 2019 |
| Předmět: |
Pore Forming Cytotoxic Proteins
Yersinia Infections Yersinia pestis Cross Protection Immunology polymorphism Mice vaccine Animals Original Research Antigens Bacterial Mice Inbred BALB C Lactoccus lactis Virulence Vaccination immune escape Antibodies Bacterial plague Lactococcus lactis Disease Models Animal LcrV Yersinia pseudotuberculosis Bacterial Vaccines bacteria Female probiotic |
| Zdroj: | Frontiers in Immunology |
| ISSN: | 1664-3224 |
| Popis: | Yersinioses caused by Yersinia pestis, Yersinia pseudotuberculosis, and Yersinia enterocolitica are significant concerns in human and veterinary health. The link between virulence and the potent LcrV antigen has prompted the latter's selection as a major component of anti-Yersinia vaccines. Here, we report that (i) the group of Yersinia species encompassing Y. pestis and Y. pseudotuberculosis produces at least five different clades of LcrV and (ii) vaccination of mice with an LcrV-secreting Lactococcus lactis only protected against Yersinia strains producing the same LcrV clade as that of used for vaccination. By vaccinating with engineered LcrVs and challenging mice with strains producing either type of LcrV or a LcrV mutated for regions of interest, we highlight key polymorphic residues responsible for the absence of cross-protection. Our results show that an anti-LcrV-based vaccine should contain multiple LcrV clades if protection against the widest possible array of Yersinia strains is sought. |
| Databáze: | OpenAIRE |
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