Developmental conditioning of endothelium-derived hyperpolarizing factor-mediated vasorelaxation
| Autor: | Rebecca, Stead, Moji G, Musa, Claire L, Bryant, Stuart A, Lanham, David A, Johnston, Richard, Reynolds, Christopher, Torrens, Paul A, Fraser, Geraldine F, Clough |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Nitric Oxide Synthase Type III Small-Conductance Calcium-Activated Potassium Channels Vasodilator Agents Down-Regulation developmental conditioning Diet High-Fat Nitric Oxide Real-Time Polymerase Chain Reaction Biological Factors Mice Norepinephrine intermediate conductance calcium-activated potassium channels Pregnancy ORIGINAL PAPERS: Pathophysiological aspects endothelium derived hyperpolarizing factor Animals Vasoconstrictor Agents RNA Messenger Intermediate-Conductance Calcium-Activated Potassium Channels Acetylcholine Diet Mesenteric Arteries Vasodilation maternal obesity Arterioles Vasoconstriction Prenatal Exposure Delayed Effects Hypertension Female microvasculature |
| Zdroj: | Journal of Hypertension |
| ISSN: | 1473-5598 |
| Popis: | Objectives: The endothelium maintains vascular homeostasis through the release of endothelium-derived relaxing factors (EDRF) and endothelium-derived hyperpolarization (EDH). The balance in EDH : EDRF is disturbed in cardiovascular disease and may also be susceptible to developmental conditioning through exposure to an adverse uterine environment to predispose to later risk of hypertension and vascular disease. Methods: Developmentally conditioned changes in EDH : EDRF signalling pathways were investigated in cremaster arterioles (18–32 μm diameter) and third-order mesenteric arteries of adult male mice offspring of dams fed either a fat-rich (high fat, HF, 45% energy from fat) or control (C, 10% energy from fat) diet. After weaning, offspring either continued on high fat or were placed on control diets to give four dietary groups (C/C, HF/C, C/HF, and HF/HF) and studied at 15 weeks of age. Results: EDH via intermediate (IKCa) and small (SKca) conductance calcium-activated potassium channels contributed less than 10% to arteriolar acetylcholine-induced relaxation in in-situ conditioned HF/C offspring compared with ∼60% in C/C (P |
| Databáze: | OpenAIRE |
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