Detection of mutant k-ras sequences in the urine of cancer patients

Autor:	O I, Serdyuk, I V, Botezatu, V P, Shelepov, G I, Potapova, R P, Alekhina, Y K, Molyaka, V S, Anan'ev, V I, Knysh, O S, Melkonyan, S R, Umanskii, A V, Lichtenshtein
Rok vydání:	2000
Předmět:	Genes ras Colonic Neoplasms Mutation Humans Codon Polymerase Chain Reaction
Zdroj:	Bulletin of experimental biology and medicine. 131(3)
ISSN:	0007-4888
Popis:	DNA fragments from apoptotic cells crossing the renal barrier retain their matrix functions, which allows PCR identification of mutant sequences in excreted DNA. We investigated the possibility of detecting k-ras mutations in urinary DNA of tumor patients (colon cancer). In some patients with k-ras codon 12 mutations in tumor cell DNA the same changes were detected in the urinary DNA. The possibility of using this approach for early diagnosis and monitoring of tumors is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::d0465f4bca76db6b9d87dd7217dcb965 https://pubmed.ncbi.nlm.nih.gov/11427923 Zobrazit plný text záznamu Full text from SpringerLink