| Autor: |
Alexander, Vallmitjana, Paola, Lepanto, Florencia, Irigoin, Leonel, Malacrida |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Methods and applications in fluorescence. 11(1) |
| ISSN: |
2050-6120 |
| Popis: |
Hyperspectral imaging (HSI) is a paramount technique in biomedical science, however, unmixing and quantification of each spectral component is a challenging task. Traditional unmixing relies on algorithms that need spectroscopic parameters from the fluorescent species in the sample. The phasor-based multi-harmonic unmixing method requires only the empirical measurement of the pure species to compute the pixel-wise photon fraction of every spectral component. Using simulations, we demonstrate the feasibility of the approach for up to 5 components and explore the use of adding a 6th unknown component representing autofluorescence. The simulations show that the method can be successfully used in typical confocal imaging experiments (with pixel photon counts between 10 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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