The role of ischemic preconditioning in the expression of apoptosis-related genes in a rat model of intestinal ischemia-reperfusion injury 1
| Autor: | de Miranda, Celina Teresa Castelo Branco Couto, Fagundes, Djalma José, de Miranda, Edinaldo, Simões, Ricardo Santos, Carbonel, Adriana Aparecida Ferraz, Florencio-Silva, Rinaldo, Taha, Murched Omar |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Down-Regulation Endothelial Cells Gene Expression Reproducibility of Results Apoptosis Real-Time Polymerase Chain Reaction Constriction Rats Random Allocation Jejunum Experimental Surgery Mesenteric Artery Superior Reference Values Mesenteric Ischemia Reperfusion Injury parasitic diseases Reperfusion Animals cardiovascular diseases Rats Wistar Ischemic Preconditioning Apoptosis Regulatory Proteins ORIGINAL ARTICLE |
| Zdroj: | Acta Cirúrgica Brasileira |
| ISSN: | 1678-2674 0102-8650 |
| Popis: | Purpose: To analyze the effects of ischemic preconditioning (IPC) in the expression of apoptosis-related genes in rat small intestine subjected to ischemia and reperfusion. Methods: Thirty anesthetized rats underwent laparotomy and were drive into five groups: control (CG); ischemia (IG); ischemia and reperfusion (IRG); IPC and ischemia (IG+IPC); IPC and ischemia and reperfusion (I/RG+IPC). Intestinal ischemia was performed by clamping the superior mesenteric artery for 60 minutes, whereas reperfusion lasted for 120 minutes. IPC was carried out by one cycle of 5 minutes of ischemia followed by 10 minutes of reperfusion prior to the prolonged 60-minutes-ischemia and 120-minutes-reperfusion. Thereafter, the rats were euthanized and samples of small intestine were processed for histology and gene expression. Results: Histology of myenteric plexus showed a higher presence of neurons presenting pyknotic nuclei and condensed chromatin in the IG and IRG. IG+IPC and I/RG+IPC groups exhibited neurons with preserved volume and nuclei, along with significant up-regulation of the anti-apoptotic protein Bcl2l1 and down-regulation of pro-apoptotic genes. Moreover, Bax/Bcl2 ratio was lower in the groups subjected to IPC, indicating a protective effect of IPC against apoptosis. Conclusion: Ischemic preconditioning protect rat small intestine against ischemia/reperfusion injury, reducing morphologic lesions and apoptosis. |
| Databáze: | OpenAIRE |
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