[Molecular cloning and the expression of the genes of amino acid biosynthesis of Corynebacterium glutamicum in Escherichia coli cells]

Autor:	O Iu, Beskrovnaia, M Iu, Fonshteĭn, L G, Kolibaba, N K, Iankovskiĭ, V G, Debabov
Rok vydání:	1988
Předmět:	DNA Bacterial Threonine Lysine Genetic Complementation Test Genetic Vectors Nucleic Acid Hybridization Chromosomes Bacterial Corynebacterium Gene Expression Regulation Genes Bacterial Escherichia coli Bacteriophages Amino Acids Cloning Molecular Plasmids
Zdroj:	Genetika. 24(7)
ISSN:	0016-6758
Popis:	Cloning of genes for threonine and lysine biosynthesis from Corynebacterium glutamicum was performed in Escherichia coli cells using the plasmid vector lambda pSL5. The cloned genes are identified via complementation of thrB and lysA mutations. The gene complementing thrB of E. coli is located within a 4.1 kb EcoRI fragment of C. glutamicum chromosomal DNA. All the recombinant phasmids complementing the lysA gene of E. coli contain common 2.2 kb and 3.3 kb EcoRI C. glutamicum DNA fragments. The cloned DNA fragments hybridize with identical EcoRI fragments of C. glutamicum chromosomal DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::cee8dd30bc8a9180077c0dd86d26fec4 https://pubmed.ncbi.nlm.nih.gov/3141247 Zobrazit plný text záznamu