Identification of sites of degradation in a therapeutic monoclonal antibody by peptide mapping

Autor:	D J, Kroon, A, Baldwin-Ferro, P, Lalan
Rok vydání:	1992
Předmět:	Cross-Linking Reagents Molecular Sequence Data Temperature Amino Acid Sequence Cysteine Asparagine Oxidation-Reduction Peptide Mapping Chromatography High Pressure Liquid Muromonab-CD3
Zdroj:	Pharmaceutical research. 9(11)
ISSN:	0724-8741
Popis:	A peptide mapping procedure was developed to locate regions of a monoclonal antibody, OKT3, that undergo chemical modification as the molecule degrades upon storage. The structures of these peptide degradation products were investigated. Deamidation at specific asparagine residues and oxidation of a cysteine and several methionines were found to be major routes of OKT3 degradation. A unique chain cross-linked degradation product was also observed and characterized. Changing the storage conditions of the antibody affected the relative distribution of degradation products. These results were useful in the development of more stable formulations for OKT3, and the methods can be used in the characterization of other monoclonal antibodies intended for therapeutic use.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::ceb720f3ab2ab78d1984fffc3c267645 https://pubmed.ncbi.nlm.nih.gov/1475223 Zobrazit plný text záznamu Full text from SpringerLink