| Autor: |
Olivia, Abigail Robles-Rodríguez, José Juan, Pérez Trujillo, José Alberto, Barrón Cantú, Abigail, Torres Cerda, Yolanda, Gutiérrez Puente, Aracely, García García, Humberto, Rodríguez Rocha, Arnulfo, Villanueva Olivo, Laura, M Zavala Flores, Odila, Saucedo Cárdenas, Roberto, Montes de Oca Luna, María, de Jesús Loera Arias |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Journal of B.U.ON. : official journal of the Balkan Union of Oncology. 25(1) |
| ISSN: |
2241-6293 |
| Popis: |
To compare the antitumor effect of adenoviruses that express mutant variants of the protein E7 from HPV-16 fused to calreticulin.Recombinant adenoviruses were generated to express calreticulin fused to mutant versions of E7 (CRT/E7m and CRT/E7dm). Western blot and immunofluorescence assays were made to demonstrate protein expression. Antitumor assays were performed in C57BL6 mice injected with TC-1 cell line.When HEK293 cells were infected with these adenoviruses, we detected that all the recombinant proteins were expressed at endoplasmic reticulum, as expected. Next, the antitumor effect was tested on a murine tumor model established by inoculation of TC-1 cell line. We detected that both Ad CRT/E7m and Ad CRT/E7dm were capable of reducing the antitumor volume when compared to Ad LacZ, which was used as negative control. No significant difference was observed when compared to Ad CRT/E7, a positive control.Here we demonstrated that the mutant versions of E7 HPV-16 fused to calreticulin generate similar antitumor effect than the wild type version. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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