Mechanisms of K(+) induced renal vasodilation in normo- and hypertensive rats in vivo
| Autor: | L, Magnusson, C M, Sorensen, T H, Braunstein, N-H, Holstein-Rathlou, M, Salomonsson |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Barium Compounds Fluorescent Antibody Technique Kidney Adaptation Physiological Rats Renal Circulation Rats Sprague-Dawley Vasodilation Chlorides Rats Inbred SHR Potassium Animals Vascular Resistance Enzyme Inhibitors Potassium Channels Inwardly Rectifying Sodium-Potassium-Exchanging ATPase Ouabain |
| Zdroj: | Acta physiologica (Oxford, England). 202(4) |
| ISSN: | 1748-1716 |
| Popis: | We investigated the mechanisms behind K(+) -induced renal vasodilation in vivo in normotensive Sprague-Dawley (SD) rats and spontaneously hypertensive rats (SHR).Renal blood flow (RBF) was measured utilizing an ultrasonic Doppler flow probe. Renal vascular resistance (RVR) was calculated as the ratio of mean arterial pressure (MAP) and RBF (RVR = MAP/RBF). Test drugs were introduced directly into the renal artery. Inward rectifier K(+) (K(ir) ) channels and Na(+) ,K(+) -ATPase were blocked by Ba(2+) and ouabain (estimated plasma concentrations ∼20 and ∼7 μm) respectively.Confocal immunofluorescence microscopy demonstrated K(ir) 2.1 channels in pre-glomerular vessels of SD and SHR. Ba(2+) caused a transient (6-13%) increase in baseline RVR in both SD and SHR. Ouabain had a similar effect. Elevated renal plasma [K(+) ] (∼12 mm) caused a small and sustained decrease (5-13%) in RVR in both strains. This decrease was significantly larger in SHR than in SD. The K(+) -induced vasodilation was attenuated by Ba(2+) in control SD and SHR and by ouabain in SD. Nitric oxide (NO) blockade using l-NAME treatment increased MAP and decreased RBF in both rat strains, but did not affect the K(+) -induced renal vasodilation.K(+) -induced renal vasodilation is larger in SHR, mediated by K(ir) channels in SD and SHR, and in addition, by Na(+) ,K(+) -ATPase in SD. In addition, NO is not essential for K(+) -induced renal vasodilation. |
| Databáze: | OpenAIRE |
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