Effect of CPoint, EndoSequence BC, and Gutta-percha Points on Viability and Gene Expression of Periodontal Ligament Fibroblasts

Autor: Claudia Bosio, Meneses, Alessandra Fonseca, Gambini, Lucas Tofanello, Olivi, Marcelo Dos, Santos, Carla Renata, Sipert
Rok vydání: 2019
Předmět:
Zdroj: European Endodontic Journal
ISSN: 2548-0839
Popis: Objective: This study aimed to investigate the cytotoxic and biomodulatory potential of conventional gutta-percha (CGP) points, gutta-percha points containing bioceramics (BC), and CPoint polymer (CP) points on periodontal ligament (PDL) cells in vitro. Methods: PDL fibroblasts were cultured and stimulated with extracts of CGP, BC, and CP in serial dilutions to evaluate cell viability using MTT assay. Next, the 1:5 dilution was used to stimulate the cells for 72 h to assess the gene expression of type I collagen (COL-1) and cement protein 1 (CEMP-1), by reverse transcription followed by quantitative PCR. Data were statistically analyzed using one-way analysis of variance (ANOVA) (P
Databáze: OpenAIRE