Autor: |
Claudia Bosio, Meneses, Alessandra Fonseca, Gambini, Lucas Tofanello, Olivi, Marcelo Dos, Santos, Carla Renata, Sipert |
Rok vydání: |
2019 |
Předmět: |
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Zdroj: |
European Endodontic Journal |
ISSN: |
2548-0839 |
Popis: |
Objective: This study aimed to investigate the cytotoxic and biomodulatory potential of conventional gutta-percha (CGP) points, gutta-percha points containing bioceramics (BC), and CPoint polymer (CP) points on periodontal ligament (PDL) cells in vitro. Methods: PDL fibroblasts were cultured and stimulated with extracts of CGP, BC, and CP in serial dilutions to evaluate cell viability using MTT assay. Next, the 1:5 dilution was used to stimulate the cells for 72 h to assess the gene expression of type I collagen (COL-1) and cement protein 1 (CEMP-1), by reverse transcription followed by quantitative PCR. Data were statistically analyzed using one-way analysis of variance (ANOVA) (P |
Databáze: |
OpenAIRE |
Externí odkaz: |
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