Thermal stability and aggregation of creatine kinase from rabbit skeletal muscle. Effect of 2-hydroxypropyl-beta-cyclodextrin

Autor:	Olga I, Maloletkina, Kira A, Markossian, Lyubov V, Belousova, Sergey Yu, Kleimenov, Victor N, Orlov, Valentina F, Makeeva, Boris I, Kurganov
Rok vydání:	2009
Předmět:	Protein Denaturation Light beta-Cyclodextrins Temperature 2-Hydroxypropyl-beta-cyclodextrin Enzyme Activation Kinetics Spectrometry Fluorescence Enzyme Stability Animals Scattering Radiation Rabbits Particle Size Muscle Skeletal Creatine Kinase Protein Binding
Zdroj:	Biophysical chemistry. 148(1-3)
ISSN:	1873-4200
Popis:	Effect of 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) on thermal aggregation of creatine kinase from rabbit skeletal muscle (RMCK) at 48 degrees C has been studied using dynamic light scattering. An increase in the duration of the lag period on the kinetic curves of aggregation, registered as an increment of the light scattering intensity in time, has been observed in the presence of HP-beta-CD. It has been shown that the initial parts of the dependences of the hydrodynamic radius (R(h)) of the protein aggregates on time follow the exponential law. The reciprocal value of parameter t(2R) (t(2R) is the time interval over which the R(h) value is doubled) was used to characterize the rate of aggregation. A 10-fold decrease in the 1/t(2R) value was observed in the presence of 76mM HP-beta-CD. Judging from the data on the kinetics of RMCK inactivation and the data on differential scanning calorimetry of RMCK, HP-beta-CD does not affect the rate of RMCK unfolding.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::cd79323282639ad7505c3e96aad4baec https://pubmed.ncbi.nlm.nih.gov/20378240 Zobrazit plný text záznamu Full Text from ScienceDirect