| Autor: |
V V, Ovod, E L, Zdorovenko, A S, Shashkov, N A, Kocharova, Iu A, Knirel' |
| Rok vydání: |
2005 |
| Předmět: |
|
| Zdroj: |
Mikrobiologiia. 73(6) |
| ISSN: |
0026-3656 |
| Popis: |
Novel O-serotypes were revealed among Pseudomonas syringae pv. garcae strains by using a set of mouse monoclonal antibodies specific to the lipopolysaccharide O-polysaccharide. Structural studies showed that the O-polysaccharide of P. syringae pv. garcae NCPPB 2708 is a hitherto unknown linear L-rhamnan lacking strict regularity and having two oligosaccharide repeating units I and II, which differ in the position of substitution in one of the rhamnose residues and have the following structures: I: --3)-alpha-L-Rha-(1 --2)-alpha-L-Rha-(1 --2)-alpha-L-Rha-(1 --3)-alpha-L-Rha-(1 --; II: --3)-alpha-L-Rha-(1 --3)-alpha-L-Rha-(1 --2)-alpha-L-Rha-(1 --3)-alpha-L-Rha-(1 --. The branched polysaccharides of P. syringae pv. garcae ICMP 8047 and NCPPB 588 have the same L-rhamnan backbone with repeating units I and II and a lateral chain of (alpha1 --4)- or (alpha1 --3)-linked residues of 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc). Several monoclonal antibody epitopes associated with the L-rhamnan backbone or the lateral alpha-D-Fuc3NAc residues were characterized. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
