Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes

Autor:	R Z, Machado, C A, Valadão, W R, Melo, A C, Alessi
Rok vydání:	1994
Předmět:	Microscopy Electron Erythrocytes Babesia bovis Animals Antibodies Protozoan Babesia Fluorescent Antibody Technique Cattle Ammonium Chloride
Zdroj:	Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. 27(11)
ISSN:	0100-879X
Popis:	1. We describe the isolation of viable merozoites from erythrocytes infected with Babesia bovis or Babesia bigemina organisms by ammonium chloride lysis. 2. Parasite morphology was examined by both light and transmission electron microscopy. Erythrocyte-free parasites maintain their viability and infectivity, retain their antigenicity and are suitable for use in the indirect fluorescent antibody assay.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::cd5ce2a639a5c88ae0afea915a202d5b https://pubmed.ncbi.nlm.nih.gov/7549981 Zobrazit plný text záznamu