| Autor: |
Rui-Shu, Li, Ming-Chen, Ni, Hui-Jun, Zhu, Qin-Qin, Ma, Min, Fu, Ping, Lu |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 29(1) |
| ISSN: |
1009-2137 |
| Popis: |
To establish quantitative surface plasmon resonance (SPR) assay for antibodies against human platelet antigen-1a (HPA-1a).Recombinant protein was fixed on the chip surface by amino coupling method. SPR assay was used to detect the standard antibodies against HPA-1a at different conceatration. The optimal experimental parameters were determined, and standard curves were constructed with linear regression. Moreover, the sensitivity, specificity, accuracy and precision of the assay were evaluated.The quantitative SPR assay for HPA-1a antibodies was established. The determination ranges were 0-20 IU, with accuracy (recovery rate) was 97.75%-103.08%. The intra-assay precision [coefficients of variation (CV)] was 3.53%-4.29%, and the inter-assay precision (CV) was 2.08%-4.40%. For specificity test, several kinds of monoclonal and human antibodies against platelet membrane protein were tested and no positive result was observed.The established quantitative SPR assay for HPA-1a antibodies shows good sensitivity, specificity, accuracy and precision, and this rapid and simple method provides a new reference method for scientific research and clinical antibody detection.HPA-1a抗体SPR技术定量检测方法的建立.建立针对人类血小板抗原（HPA）-1a抗体的表面等离子体共振（SPR）技术定量检测方法.以氨基偶联法在芯片表面偶联重组蛋白，采用SPR方法检测不同浓度的标准抗血浆样本，优化实验条件，在检测范围内绘制标准曲线，并分析其敏感性、特异性、准确性和精密度.利用SPR技术建立针对HPA-1a抗体的定量检测方法，检测范围为0-20 IU，准确性（回收率）为97.75%-103.08%，批内精密度[变异系数（CV）]为3.53%-4.29%；批间精密度（CV）为2.08%-4.40%。特异性实验中，以不同的血小板膜蛋白单抗和含相应抗体的参比血浆进行检测，均未见明显阳性结果.建立的针对HPA-1a抗体的SPR技术定量检测方法具有较高的敏感性、特异性、准确性和精密度，且操作简便快速，这为科研和临床相关抗体的检测提供了一种新的参考方法. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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