| Autor: |
Lei, Liu, Sa, Shi, Hong-Zhu, Li, Hong, Li, Chang-Qing, Xu |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology. 32(3) |
| ISSN: |
1000-6834 |
| Popis: |
To study the effect of excited dopamine type I receptor on the production of nitric oxide/nitric oxide synthase(NO/NOS)in ox-LDL activated THP-1 cells and the possible mechanism.Cultured THP-1 cells activated by PMA were randomly assigned in the following groups:control group (control), oxidized low density lipoprotein group (ox-LDL), dopamine receptor 1(DR1) agonist group (SKF), DR1 antagonist group (SCH), ERK blocker group (PD98059). Oil Red O staining was used to identify the accumulation of cellular lipid. The levels of NO and NOS in the supernatant of THP-1 were assayed by nitrate reductase method. The protein expression of DR1, p-ERK and ERK were obtained by Western blot and immunity fluorescence.After 48 h of incubation of ox-LDL, accumulation of lipid in the cytoplasm was found in most THP-1 cells. Compared with control group, DR1 protein expression was reduced in ox-LDL-induced cells(Activation of DR1 can inhibit the production of NO/NOS in ox-LDL-induced THP-1 cells, which may be related with ERK pathway. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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