| Autor: |
Shi-Bin, Song, Zhi-Zhong, Jing, Guo-Hu, Chen, Xiao-Xiao, Wang, Rui-Qian, Zong |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 23(5) |
| ISSN: |
1000-3061 |
| Popis: |
The porcine IL-18 gene was amplified from recombinant plasmid pGEM-IL-18 by PCR, then the pPIC9K-IL-18 of fusion expression vector was constructed by inserting IL-18 fragment,and was transformed to GS115 by electroporation, multi-copy recombinant strains were screened by G418. The expression of recombinant fusion protein was induced by methanol, SDS-PAGE was used to analyze expression product, fusion protein was purified by Sephadex G-100 column, bioactivity of IL-18 was measured by MTT assays. Experiment results show fusion protein of pIL-18 secreted by GS115,expression reaches the secretion peak of 160 mg/L at 72 h. We have expressed and purified successfully the recombinant pIL-18 with obvious biological activity in Pichia pastoris. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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