Use of fluorescent europium chelates as labels in microscopy allows glutaraldehyde fixation and permanent mounting and leads to reduced autofluorescence and good long-term stability

Autor:	L, Sevéus, M, Väisälä, I, Hemmilä, H, Kojola, G M, Roomans, E, Soini
Rok vydání:	1994
Předmět:	Fixatives Tissue Fixation Europium Microscopy Fluorescence Antigens Neoplasm Glutaral Colonic Neoplasms Fluoroimmunoassay Antibodies Monoclonal Humans Chelating Agents Fluorescent Dyes
Zdroj:	Microscopy research and technique. 28(2)
ISSN:	1059-910X
Popis:	In the present study europium chelates were introduced as alternative fluorescent labels for microscopy and their effect on enhanced autofluorescence caused by the glutaraldehyde fixative was investigated. Glutaraldehyde fixation was used to stabilize the cells for a permanent mount after the immunocytochemical reaction. The europium signal in time-resolved fluorescence microscopy was shown to be free of autofluorescence when strong cross-linking fixation with glutaraldehyde was used and the signal-to-background ratio obtained was 2,400 or better. It was also shown that the europium signal was stable in daylight and at room temperature. Fluorescent europium chelate used in this experiment provides excellent contrast and long-term stability for the samples with glutaraldehyde fixation and permanent mounting.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::ccb4ce5fb9a187004777909cf4f932fe https://pubmed.ncbi.nlm.nih.gov/8054664 Zobrazit plný text záznamu Plný text