Methods for animal satellite cell culture under a variety of conditions

Autor: N M, Burton, J, Vierck, L, Krabbenhoft, K, Bryne, M V, Dodson
Rok vydání: 2000
Předmět:
Zdroj: Methods in cell science : an official journal of the Society for In Vitro Biology. 22(1)
ISSN: 1381-5741
Popis: Primary and clonal culture systems have been devised and refined for animal-derived satellite cells. Satellite cell (SC) culture development includes efficient cell isolation techniques, establishment of effective plating and growth conditions, formulation of media requirements and thorough evaluation of experimental limitations. As the field of muscle cell culture has expanded, the number of animal species from which satellite cells have been isolated has increased. The focus of this paper is to compare and contrast SC culture conditions presently used by a variety of researchers and to introduce a new source of SC from wapiti (elk).
Databáze: OpenAIRE