Genotyping of clinical and chicken isolates of Campylobacter jejuni and Campylobacter coli

Autor: S I, Smith, D K, Olukoya, A J, Fox, A O, Coker
Rok vydání: 2000
Předmět:
Zdroj: Cytobios. 103(403)
ISSN: 0011-4529
Popis: Genomic DNA from 58 strains of Campylobacter made up of 48 Campylobacter jejuni and ten Campylobacter coli were digested with Sma I and analysed by pulsed-field gel electrophoresis (PFGE). The cleavage of DNA by Sma I gave 22 distinct hybridization patterns. The two Campylobacter species were subtyped by PFGE. The average genomic size for C. jejuni by Sma I digestion was 1.73 Mb, while that of C. coli gave 1.7 Mb. Results from this study indicate that PFGE analysis by Sma I digested genomic DNA provides a reliable means of differentiating between and within species of Campylobacter and provides a practical approach to epidemiological studies of Campylobacter.
Databáze: OpenAIRE