[The obtaining and analysis of physiological activity of secreted proteins of Noggin family]
| Autor: | F M, Eroshkin, A V, Baĭramov, O V, Aver'ianova, E A, Solov'eva, M V, Serebriakova, A G, Zaraiskiĭ, N Iu, Martynova |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Intracellular Signaling Peptides and Proteins
Embryonic Development Gene Expression Regulation Developmental Bone Morphogenetic Protein 4 Xenopus Proteins Recombinant Proteins Xenopus laevis Transforming Growth Factor beta Animals Amino Acid Sequence RNA Messenger Carrier Proteins Protein Binding Signal Transduction |
| Zdroj: | Bioorganicheskaia khimiia. 39(2) |
| ISSN: | 0132-3423 |
| Popis: | We have developed methods for producing recombinant proteins of Noggin family (Noggin1 and Noggin2 of the Xenopus laevis frog) that can interact with BMP factors of TGF-beta superfamily. The genetic constructs which allow one to effectively obtain Noggin1 and Noggin2 from synthetic mRNA microinjected into Xenopus laevis early embryos, as well as in the prokaryotic expression system, were generated. The obtained proteins contain three Myc-tag epitopes on their N-terminus. This allow one to compare the expression levels of Noggin1 and Noggin 2 constructs, to purify them on the affine immunosorbent and to show the activity of Noggin proteins by analyzing their ability to bind BMP4 factor TGF-beta surperfamily by co-immunoprecipitation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|