PCR-Restriction Fragment Length Polymorphism Analysis of a Diagnostic 452-Base-Pair DNA Fragment Discriminates between Cryptosporidium parvum and C. meleagridis and between C. parvum Isolates of Human and Animal Origin
| Autor: | Guyot, K., Follet-Dumoulin, A., Recourt, C., Lelièvre, E., Cailliez, J. C., Dei-Cas, E. |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Cryptosporidium parvum
Base Sequence animal diseases Molecular Sequence Data Cattle Diseases Cryptosporidiosis Cryptosporidium Public Health Microbiology DNA Protozoan DNA Ribosomal Polymerase Chain Reaction digestive system diseases parasitic diseases RNA Ribosomal 18S Animals Humans Cattle Base Pairing Polymorphism Restriction Fragment Length |
| Popis: | Genomic DNAs from human Cryptosporidium isolates previously typed by analysis of the 18S ribosomal DNA locus (Cryptosporidium parvum bovine genotype, C. parvum human genotype, Cryptosporidium meleagridis, and Cryptosporidium felis) were used to amplify the diagnostic fragment described by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg., 45:688-694, 1991). The obtained 452-bp amplified fragments were sequenced and aligned with the homologous Cryptosporidium wrairi sequence. Polymorphism was exploited to develop a restriction fragment length polymorphism method able to discriminate Cryptosporidium species and C. parvum genotypes. |
| Databáze: | OpenAIRE |
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