Cultured tumor cells of murine submandibular gland origin: a model to investigate pHi regulation of salivary cells
| Autor: | E, Trzaskawka, J, Vigo, J C, Egea, M C, Goldsmith, J M, Salmon, D D, De Periere |
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| Rok vydání: | 2000 |
| Předmět: |
Acid-Base Equilibrium
Ion Transport Microscopy Video Sodium-Hydrogen Exchangers Sodium-Potassium-Chloride Symporters Submandibular Gland Biological Transport Active Intracellular Membranes Hydrogen-Ion Concentration Models Biological Ammonium Chloride Antiporters Mice Tumor Cells Cultured Animals Fluorometry Chloride-Bicarbonate Antiporters Carrier Proteins Hydrogen |
| Zdroj: | European journal of oral sciences. 108(1) |
| ISSN: | 0909-8836 |
| Popis: | Intracellular pH (pHi) and several ion transport mechanisms in cultured murine salivary gland cells (SCA-9) were studied using a videomicrofluorometric method and the H+-specific probe C-SNARF-1. The aim of this study was to test SCA-9 cells' pHi regulation mechanisms and evaluate if this cell line is representative of submandibular gland cells. Resting pHi in unstimulated cells was estimated to be 7.17+/-0.07. To investigate the presence of Na+/H+ and Cl-/HCO3- antiports as well as Na+/K+/2Cl- symports in SCA-9 cells, we used different specific blockers, dimethyl-amiloride, disulfonic stilbene, bumetanide and furosemide. In order to study SCA-9 cell capacity to regulate their pHi in response to alkaline and acid loads, we applied the NH4Cl prepulse method to all these blockers. The results showed that SCA-9 cells possess both antiports and symports involved in pHi regulation, and that this cell line can be used as a convenient model to study pHi regulatory mechanisms in salivary cells. |
| Databáze: | OpenAIRE |
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