| Autor: |
Elisabetta, Coppi, Federica, Cherchi, Erica, Sarchielli, Irene, Fusco, Giulia, Guarnieri, Pasquale, Gallina, Renato, Corradetti, Felicita, Pedata, Gabriella B, Vannelli, Anna Maria, Pugliese, Annamaria, Morelli |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Journal of neurochemistryREFERENCES. 157(4) |
| ISSN: |
1471-4159 |
| Popis: |
The Nucleus Basalis of Meynert (NBM) is the main source of cholinergic neurons in the basal forebrain to be crucially involved in cognitive functions and whose degeneration correlates with cognitive decline in major degenerative pathologies as Alzheimer's and Parkinson's diseases. However, knowledge concerning NBM neurons derived from human brain is very limited to date. We recently characterized a primary culture of proliferating neuroblasts isolated from the human fetal NBM (hfNBM) as immature cholinergic neurons expressing the machinery to synthetize and release acetylcholine. Here we studied in detail electrophysiological features and cholinergic effects in this cell culture by patch-clamp recordings. Our data demonstrate that atropine-blocked muscarinic receptor activation by acetylcholine or carbachol enhanced I |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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