| Autor: |
Ting, Luo, Xue-Qing, Ou-Yang, Li-Tao, Yang, Yang-Rui, Li, Xiu-Peng, Song, Ge-Min, Zhang, Yi-Jing, Gao, Wei-Xing, Duan, Qianli, An |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Journal of basic microbiology. 56(8) |
| ISSN: |
1521-4028 |
| Popis: |
N2 -fixing bacteria belonging to the genus Raoultella of the family Enterobacteriaceae are widely associated with plants. Raoultella sp. strain L03 was isolated from surface-sterilized sugarcane roots. In this study, we inoculated the strain L03 to microbe-free micropropagated plantlets of the main sugarcane cultivar ROC22 grown in Guangxi, China and determined N2 -fixation and association between strain L03 and sugarcane plants. Inoculation of strain L03 increased plant biomass, total N, N concentration and chlorophyll, and relieved N-deficiency symptoms of plants under an N-limiting condition. An (15) N isotope dilution assay revealed (15) N isotope dilution in the inoculated sugarcane plants and incorporation of the fixed (14) N from air into chlorophyll. Moreover, a gfp-tagged and antibiotic-resistant L03 strain was reisolated from surface-sterilized sugarcane plants and was detected in plant tissues by fluorescent microscopy. This study for the first time demonstrates that a Raoultella bacterium is able to fix N2 in association with the plant host. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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