Anti-p53 antibodies in sera from patients with chronic obstructive pulmonary disease can predate a diagnosis of cancer
| Autor: | G E, Trivers, V M, De Benedetti, H L, Cawley, G, Caron, A M, Harrington, W P, Bennett, J R, Jett, T V, Colby, H, Tazelaar, P, Pairolero, R D, Miller, C C, Harris |
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| Rok vydání: | 1996 |
| Předmět: |
Aged
80 and over Male Antibodies Neoplasm DNA Mutational Analysis Immunoblotting Middle Aged Prognosis Immunohistochemistry Precipitin Tests Antibodies Immunoenzyme Techniques Case-Control Studies Neoplasms Mutation Humans Female Lung Diseases Obstructive Tumor Suppressor Protein p53 Polymorphism Single-Stranded Conformational Aged Randomized Controlled Trials as Topic Retrospective Studies |
| Zdroj: | Clinical cancer research : an official journal of the American Association for Cancer Research. 2(10) |
| ISSN: | 1078-0432 |
| Popis: | Serum anti-p53 antibodies (p53-Abs) may be surrogate markers for both p53 alterations and preclinical cancer. Ancillary to a prospective trial to abate progressive development of clinical stages of chronic obstructive pulmonary disease, we conducted a retrospective, nested case-control study. Twenty-three cases were diagnosed with cancer during the trial. Enzyme immunoassay, immunoblotting, and immunoprecipitation were used to detect p53-Abs in serum, immunohistochemistry (IHC) to detect p53 accumulation, and single-strand conformation polymorphism and DNA sequencing to detect p53 mutations in tumor samples. p53-Abs were detected by three types of assays in five (23%) of the cancer patients, 80% of whom had detectable p53-Abs before diagnosis: 2 lung cancers (7 and 6 months before), 1 prostate cancer (11 months), and 1 breast cancer (5 months). Four Ab-positive patients had IHC-positive tumors. Two of 4 Ab-positive patients and 2 of 14 Ab-negative had p53 missense mutations or base pair deletion and IHC-positive tumors. The 44 noncancer COPD controls, matched with the cancer cases for age, gender, and smoking habits, were negative for p53-Abs. These results indicate that p53-Abs may facilitate the early diagnosis of cancer in a subset of smokers with chronic obstructive pulmonary disease who are at an increased cancer risk. |
| Databáze: | OpenAIRE |
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