| Autor: |
Aleksandra E, Sikora, Richard, Tehan, Kerry, McPhail |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1839 |
| ISSN: |
1940-6029 |
| Popis: |
The development of antibiotic-resistant bacteria requires increasing research efforts in drug discovery. Vibrio cholerae can be utilized as a model gram-negative enteric pathogen in high- and medium-throughput screening campaigns to identify antimicrobials with different modes of action. In this chapter, we describe methods for the optimal growth of V. cholerae in 384-well plates, preparation of suitable microtiter natural product sample libraries, as well as their screening using measurements of bacterial density and activity of type II secretion-dependent protease as readouts. Concomitant LC-MS/MS profiling and spectral data networking of assay sample libraries facilitate dereplication of putative known and/or nuisance compounds and efficient prioritization of samples containing putative new natural products for further investigation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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