Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function
| Autor: | Zhao, Shengli, Ting, Jonathan T., Atallah, Hisham E., Qiu, Li, Tan, Jie, Gloss, Bernd, Augustine, George J., Deisseroth, Karl, Luo, Minmin, Graybiel, Ann M., Feng, Guoping |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Chromosomes
Artificial Bacterial thalamic reticular nucleus Pvalb dorsal raphe nucleus Vesicular Inhibitory Amino Acid Transport Proteins cortical fast-spiking interneurons Action Potentials Mice Transgenic transgenic mice Tryptophan Hydroxylase Hippocampus Article striatal cholinergic neurons Choline O-Acetyltransferase Mice Channelrhodopsins TPH2 VGAT Animals Nerve Tissue Parvalbumin acute brain slice BAC Neurons hippocampal interneurons fungi electrophysiology nervous system ChAT Purkinje cells ChR2(H134R) Channelrhodopsin-2 bacterial artificial chromosome ChR2-EYFP |
| Zdroj: | Nature methods |
| ISSN: | 1548-7105 1548-7091 |
| Popis: | Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function and dysfunction. We used a bacterial artificial chromosome (BAC) transgenic strategy to express the H134R variant of channelrhodopsin-2, ChR2(H134R), under the control of cell type–specific promoter elements. We performed an extensive functional characterization of the newly established VGAT-ChR2(H134R)-EYFP, ChAT-ChR2(H134R)-EYFP, Tph2-ChR2(H134R)-EYFP and Pvalb(H134R)-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action-potential firing of GABAergic, cholinergic, serotonergic and parvalbumin-expressing neuron subsets using blue light. This resource of cell type–specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior. |
| Databáze: | OpenAIRE |
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