Expression of interleukin 8 and its receptors in human colon carcinoma cells with different metastatic potentials
| Autor: | A, Li, M L, Varney, R K, Singh |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Dose-Response Relationship
Drug Reverse Transcriptase Polymerase Chain Reaction Interleukin-8 Enzyme-Linked Immunosorbent Assay Immunohistochemistry Antibodies Receptors Interleukin-8B Cell Line Receptors Interleukin-8A Gene Expression Regulation Neoplastic Cell Movement Colonic Neoplasms Cell Adhesion Animals Humans Endothelium Vascular RNA Messenger Caco-2 Cells Neoplasm Metastasis Cell Division |
| Zdroj: | Clinical cancer research : an official journal of the American Association for Cancer Research. 7(10) |
| ISSN: | 1078-0432 |
| Popis: | In the present study, we examined the expression of a multifunctional cytokine, interleukin 8 (IL-8), and its receptors, CXCR1 and CXCR2, in human colon carcinoma cells with different metastatic potentials and determined their role in modulating phenotypes associated with metastasis.IL-8, CXCR1, and CXCR2 protein and mRNA expression were examined using ELISA, immunocytochemistry, and reverse transcription-PCR in human colon carcinoma cells with different metastatic potentials. IL-8-mediated proliferation, migration, and tumor-endothelial cell interaction were analyzed.IL-8 mRNA and protein expression was very low in Caco2 cells but elevated in KM12C cells and very high in KM12L4 cells, suggesting an association between the IL-8 production and metastatic potential. Similarly, CXCR1 and CXCR2 expression was lower in Caco2 cells than in low and high metastatic KM12C and KM12L4 cells. The recombinant human IL-8 enhanced the proliferation of colon carcinoma cells. Furthermore, proliferation of low and high metastatic cells expressing different levels of IL-8 was inhibited by neutralizing antibodies to IL-8, CXCR1, and CXCR2. We observed significant differences in the invasive potential of colon carcinoma cells expressing different levels of IL-8. In addition, we observed that IL-8 modulates adhesion of tumor cells to endothelial cells in an autocrine and paracrine manner.Our present data suggest an association between constitutive expression of IL-8 and aggressiveness in human colon carcinoma cells and the possible role of IL-8 in modulating different metastatic phenotypes associated with progression and metastasis. |
| Databáze: | OpenAIRE |
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