Enhanced efficacy of transcriptionally targeted suicide gene/prodrug therapy for thyroid carcinoma with the Cre-loxP system
| Autor: | Y, Nagayama, E, Nishihara, M, Iitaka, H, Namba, S, Yamashita, M, Niwa |
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| Rok vydání: | 1999 |
| Předmět: |
Transcription
Genetic Genetic Vectors Thyroid Gland Mice Nude Genetic Therapy Transfection Thymidine Kinase Thyroglobulin Recombinant Proteins Cell Line Rats Mice Retroviridae Genes Reporter Gene Targeting Animals Humans Simplexvirus Prodrugs Thyroid Neoplasms Promoter Regions Genetic Ganciclovir Cell Line Transformed |
| Zdroj: | Cancer research. 59(13) |
| ISSN: | 0008-5472 |
| Popis: | Our recent study demonstrates the feasibility of the thyroglobulin (TG) promoter in transcriptionally targeted gene therapy for thyroid carcinomas expressing TG, albeit less effectively than the constitutive viral promoter. The present study was, therefore, designed to enhance the activity of the TG promoter with the Cre-loxP system. Our data demonstrate that the in vitro cytotoxic effect of herpes simplex virus thymidine kinase/ganciclovir obtained with the TG promoter and the Cre-loxP system is approximately 5-10-fold higher than that with the TG promoter alone. Enhanced tumor growth inhibition was also observed in in vivo tumor models. These data indicate the usefulness of the Cre-loxP system to enhance the activity of a tissue (or tumor)-specific promoter in transcriptionally targeted cancer gene therapy. |
| Databáze: | OpenAIRE |
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