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Purpose Acupuncture therapy is effective for relieving postoperative pain. Our previous study showed that electroacupuncture (EA) at Futu (LI18) and Hegu (LI4)–Neiguan (PC6) could alleviate incisional neck pain, which was related with its effect in upregulating γ-aminobutyric acid (GABA) expression in cervical (C3–6) dorsal root ganglions (DRGs); but whether its receptor subsets GABAAα2R and GABABR1 in C3–6 DRGs are involved in EA analgesia or not, it remains unknown. Materials and methods Seventy-five male Sprague Dawley rats were randomized to normal control, model, LI18, LI4–PC6, and Zusanli (ST36)–Yanglingquan (GB34) groups. The incisional neck pain model was established by making a longitudinal incision along the midline of the rats’ neck, followed by repeated mechanical stimulation. EA was applied to bilateral LI18, LI4–PC6, or ST36–GB34 for 30 minutes at 4, 24, and 48 hours after operation. The thermal pain threshold of the neck was detected by a tail-flick unit, and the C3–6 DRGs were removed for assaying the immunoactivity of substance P (SP), GABAAα2R, glial fibrillary acidic protein (GFAP; a marker of satellite glial cells [SGCs]), and GABABR1 and the expression of GABAAα2R and GABABR1 mRNA and proteins using immunofluorescence, real-time PCR, and Western blotting, respectively. Results The cervical thermal pain threshold was significantly lower in the model group than the normal group (P0.05). Immunofluorescence staining showed that GABAAα2 R expressed on SP+ neurons, and GABABR1 on SGCs. EA of LI18 and LI4–PC6 markedly suppressed the modeling-induced upregulation of the immunoactivity of SP (P |
