[Considering the oligonucleotides secondary structures at thermodynamic and kinetic analysis of the DNA-duplexes formation]
| Autor: | A A, Lomzov, D V, Pyshnyĭ |
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| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Biofizika. 57(1) |
| ISSN: | 0006-3029 |
| Popis: | The influence of secondary structures of DNA oligonucleotides on thermodynamics and kinetics at the formation of their bimolecular complexes (duplexes) has been studied. The models considering inherent secondary structures of duplex components and their influence on quantitative thermodynamic and kinetic characteristics of the duplexes have been developed. The values of thermodynamic impacts given by individual structural elements of the double helix have been shown to depend on hairpin structuring of the duplex components. The "concentration" method to consider oligonucleotides intramolecular structure with thermodynamic parameters of bimolecular duplex formation has been proposed. According to stop-flow measurements, the observed values of association and dissociation constants are influenced by the presence of inherent structures in duplex components. The influence observed is increased with the lowering of the sample temperature. The analysis of experimental data involving the developed models provides the possibility to determine "proper" kinetic constants for the helix-to-coil transition. The difference between observed and calculated rate constants can amount up to two or more orders of magnitude. |
| Databáze: | OpenAIRE |
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