The v-Rel oncoprotein increases expression from Sp1 site-containing promoters in chicken embryo fibroblasts
| Autor: | S, Sif, A J, Capobianco, T D, Gilmore |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Transcriptional Activation
Oncogene Proteins v-rel Base Sequence Transcription Genetic Sp1 Transcription Factor Molecular Sequence Data Retroviridae Proteins Oncogenic Chick Embryo Oncogenes Oncogene Proteins v-myb Proto-Oncogene Proteins c-rel Oncogene Protein pp60(v-src) DNA-Binding Proteins Proto-Oncogene Proteins p21(ras) Gene Expression Regulation Oligodeoxyribonucleotides Proto-Oncogene Proteins Animals Promoter Regions Genetic |
| Zdroj: | Oncogene. 8(9) |
| ISSN: | 0950-9232 |
| Popis: | The v-Rel oncoprotein of the avian Rev-T retrovirus is a member of a family of related transcription factors, which also includes the subunits of NF-kappa B and several other interacting cellular proteins. We show here that v-Rel specifically increased expression from a reporter plasmid containing multiple Sp1 binding sites approximately sixfold in chicken embryo fibroblasts (CEFs), even though v-Rel did not bind directly to these sites. v-Rel also increased expression from a reporter plasmid containing a human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in which the kappa B binding sites were mutated but which still contained intact Sp1 binding sites. The increase in Sp1-site transactivation does not precisely correlate with transformation by v-Rel since one non-transforming v-Rel mutant still induced expression from the Sp1 site-containing promoter. v-Rel appears to increase expression from Sp1 site-containing promoters by affecting the transactivation domain of Sp1, since v-Rel increased the activity of a Gal4-Sp1 fusion protein, which contains the Sp1 transactivation domain but lacks the Sp1 DNA-binding domain. As compared with v-Rel, c-Rel induced only a slight increase in expression from the reporter plasmid containing Sp1 sites. However, v-Ras and v-Src (but not v-Myb) induced increases in transcription from the reporter plasmid containing Sp1 sites to the same extent as v-Rel, but through pathways that appear to be independent from v-Rel. These results suggest that certain oncoproteins might increase transcription from many genes that contain Sp1 binding sites, and that this might be important for certain aspects of transformation by these proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|