| Autor: |
U, Cassens, B, Greve, K, Tapernon, B, Nave, E, Severin, W, Sibrowski, W, Göhde |
| Rok vydání: |
2002 |
| Předmět: |
|
| Zdroj: |
Vox sanguinis. 82(4) |
| ISSN: |
0042-9007 |
| Popis: |
Accurate determination of residual leucocytes [white blood cells (WBC)] in blood components is of high clinical importance. To date, several labour-intensive, time-consuming or expensive techniques have been used for this purpose.A method for the determination of residual WBC is described using a novel low-cost flow-cytometric cell counter and analyser (CCA). The DNA in WBC was stained using 4'-6-diamidino-2-phenylindole (DAPI) and WBC were automatically analysed by true volumetric counting of 200-microl samples (prepared from a 20-microl undiluted sample).Dilution experiments over a range of 0.5-50 WBC/microl showed a linearity of r = 0.998. The detection limit of this method was 0.83 WBC/microl of red blood cell concentrate (RCC) and 0.67 WBC/microl of platelet concentrate (PC), with an accuracy of 95.5%.Residual WBC (1 WBC/microl) can be accurately counted using the CCA within 2 min and at a total cost of less than euro 1 per sample. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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